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my question can more  than one meesage can be in one segment and can more than one segment be in one datagram and more than one datagram can be present in one frame
in Computer Networks
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This question is ambiguous .

Lets start with TL  ipv4 maximum data could be transmitted is 64kB (16 bit in Total Length Field See IpV4 Header )
65535 B

In Data Link Layer If we are using Ethernet It can hold upto 1500B

Network Layer Divide  Segments According to Which layer is creating bottle neck ( according to data mentioned here bottle neck will be caused by DLL . No NL takes packets of size  1460 B )

Application Layer can send any amount of data to NL and it the duty of NL to divide the data to fit into one segment .

Make a clear question with all parameters .
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